Experiments for obtaining, maintenance and propagation of certified planting material from raspberry in RIMSA Troyan.

摘要

Since 1992 in RIMSA Troyan a series of experiments have been carried out for obtaining, maintenance and preliminary propagation of virus-free planting material from raspberry. After a preliminary pomological selection and biological indexation on Rubus occidentalis and serological tests by ELISA, the complex of aphid-borne viruses and nematode-borne viruses (RRV, SLRV, TBRV, AMV) was controlled. Nuclear (pre-basic) plants were obtained from the major raspberry cultivars in our country, maintained in individual pots, sterile substrate, in isolation facilities, covered with net, as well as under in vitro conditions. A method was developed for quick preliminary propagation of the nuclear plants through root cuttings and adventitious buds. Shoot-forming capacity and vegetative characteristics and morphological features of 5 Bulgarian and 6 introduced cultivars were studied. The obtained transplants from the containers had a perfect root system and in spite of the fact that they did not attain the indicators of the Bulgarian standards with regard to the shoot thickness, they provided 100% rooting by planting in the field. Variants were elaborated to increase the efficiency of use of basic raspberry mother plantations.