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Breeding of resistant strawberry cultivars for organic fruit production - preliminary results with Botrytis cinerea.

机译:用于有机水果生产的抗性草莓品种的选育-灰葡萄孢的初步结果。

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摘要

Resistant strawberry cultivars represent an important prerequisite for organic cultivation systems to overcome high yield losses caused by fungal and bacterial diseases. To evaluate differences in susceptibility towards the fungal pathogen Botrytis cinerea Pers. (teleomorph Botryotinia fuckeliana) fruits from 13 different strawberry cultivars were artificially infected with field isolates from this pathogen. For infection, field isolates from grey mould-infected strawberries were taken and verified in a molecular approach using a PCR method. A resistance test was established by infecting fruits under standardized conditions using a defined inoculation procedure. First symptoms of grey mould occurred 1-3 dpi (days post inoculation) at 20 degrees C in the climate cabinet. Different degrees of susceptibility and resistance became apparent 6 dpi and were evaluated with a scale from 0=no fruit rot to 4=full fruit rot. Susceptible cultivars like 'Senga Sengana' reached a score of 4 at 6 dpi whereas 'Florence' showed the lowest symptoms of fruit rot with a score of 0.7 and can be considered as relatively resistant towards grey mould disease. A comprehensive genetic evaluation of numerous strawberry cultivars, breeding clones and wild types will lead to the identification of resistant genotypes.
机译:抗性草莓品种是有机栽培系统克服由真菌和细菌引起的高产量损失的重要前提。要评估对真菌病原体灰葡萄孢的敏感性。用来自该病原体的田间分离株人工感染了来自13个不同草莓品种的(teleomorph Botryotinia fuckeliana)果实。为了感染,从灰色霉菌感染的草莓中分离出田间分离物,并使用PCR方法以分子方法进行验证。通过使用定义的接种程序在标准化条件下感染水果来建立抗药性测试。在20摄氏度的气候柜中,灰霉病的最初症状出现在1-3 dpi(接种后几天)。 6 dpi出现不同程度的敏感性和抗性,并以从0 =无果腐烂到4 =全果腐烂的等级进行评估。易感品种(如“ Senga Sengana”)在6 dpi时得分为4,而“佛罗伦萨”的果实腐烂症状最低,得分为0.7,可以认为对灰霉病具有相对的抵抗力。对众多草莓品种,育种克隆和野生型进行全面的遗传评估将有助于鉴定抗性基因型。

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