Citrus germplasm enhancement via cell engineering and genetic engineering--progress report.

摘要

Citrus is an important commercial fruit tree and stable, long-term development of the citrus fruit industry depends upon continuous supply of new superior cultivars. Traditional breeding strategies have made significant progress, but improvements in citrus biotechnology will further strengthen future breeding programs. In this paper, recent progress in cell engineering and genetic engineering within our laboratory is summarized. Several somatic hybrids have been produced through either symmetric or asymmetric fusion. In addition, subprotoplasts, namely cytoplasts and microprotoplasts, have been isolated, which have great potential for transferring partial genetic components from one species to another. Furthermore, several genes involved in stress responses have been cloned from citrus or its related genus, Poncirus trifoliata, whose functions are now being elucidated. Transgenic sweet orange overexpressing MdSPDS1 and OsBADH1 have been produced via Agrobacterium-mediated transformation, with the intention of enhancing tolerance to biotic or abiotic stresses. These new techniques provide additional novel germplasms that can be exploited for future citrus germplasm enhancement.