Influence of different strains of Agrobacterium rhizogenes on induction ofhairy roots and artemisinin production in Artemisia annua

摘要

Different strains of Agrobacterium rhizogenes, viz. A(4), 15834, K-599, LBA 9402, 9365 and 9340, were evaluated for induction of transformed hairy roots in Artemisia annua using shoot-tip meristem explants. Incorporation of acetosyringone in bacterial culture and co-cultivation medium increased the frequency of hairy root induction in A. annua. Different strains of A. rhizogenes varied in their virulence for induction of hairy roots. Growth kinetics of transgenic hairy roots induced by different strains indicated a similar pattern of growth, with maximum growth occurring between 14 and 16 days. Transformed hairy root cultures showed significant differences in artemisinin content. A hairy root line induced by strain 9365 was found to contain highest amount of artemisinin (0.23%). Artemisinin content of different hairy root lines was also found to be growth-related.