Using molecular pedigree reconstruction to evaluate the long-term survival of outplanted hatchery-reared larval and juvenile northern abalone (Haliotis kamtschatkana)

摘要

The restoration of abalone (Haliotis spp.) populations through supplementation with the offspring of hatchery-spawned wild parents has been attempted in several species, with variable results. Between 2002 and 2005, the Bamfield Huu-ay-aht Community Abalone Project released 4. 5 million larvae and 152,000 juveniles of the northern abalone (Haliotis kamtschatkana) into Barkley Sound, BC. The purpose of this study was to estimate the long-term survival of outplanted abalone 3-7 years after their release and thus determine their contribution to local population densities at three different outplanting sites. We identified outplanted abalone by genotyping epipodal tentacles sampled from wild-caught abalone for seven microsatellite loci. We then used three different pedigree reconstruction programs: one that used genotypes from hatchery-reared siblings (PEDIGREE 2. 2), one that used the wild parent genotypes that were available (CERVUS 3. 0), and one that used both sources of information (COLONY 2. 0). Each program identified different but partially overlapping subsets of hatchery-outplanted offspring. From this we inferred that up to 26% of the individuals sampled at the main outplanting site were from hatchery spawnings. Despite this large contribution of hatchery-reared stock, the density of mature abalone at each site was below the level required for successful fertilization. More intensive outplanting efforts might increase population densities of this broadcast spawner above this minimum sustainable level. However, for supplementation to be successful, other factors that could reduce outplanted juvenile survival, including the low genetic diversity of hatchery-produced offspring and the low habitat quality of some outplanting sites, need to be monitored.