Protein-Free Culture of Sf9 Cells: Using a Disposable, Stirred-Tank Bioreactor

摘要

The development of insect cell culture production systems has produced opportunities for the expression of recombinant proteins for research and therapeutic applications in a non-mammalian cell culture environment. Various insect cell lines have beendeveloped for the baculovirus expression vector system (BEVS). Development of stable transfected Drosophila and lepidopteran cell lines has offered a nonlytic culture for the production of recombinant proteins from insect cells.Spodoptera frugiperda Sf9 cells have been successfully adapted to suspension culture and have become an attractive platform for industrial-scale cell culture, lending them for use in stirred-tank bioreactors, including single-use bioreactors (S.U.B.s). S.U.B. vessels reduce capital costs, cleaning, maintenance, and installation and validation time in comparison with traditional, stainless-steel vessels.