Studying the Effect of Dimebon and NT-1505 on Microviscosity of Mice Brain Synaptosomal Membranes in vivo by EPR Spin Labeling Method

摘要

In this work membrane fluidity alterations in synaptosomes, isolated from mice brain tissue, at chronic injection of neuroprotectors Dimebon and NT-1505 in vivo were studied. Membrane microviscosity was measured by electron paramagnetic resonance spin labeling of 2,2,6,6-tetramethyl-4-capryloyl-oxylpiperidine-1-oxyl (lipid probe) and 5,6-benzo-2,2,6,6-tetramethyl-1,2,3,4-tetrahydro-γ-carboline-3-oxyl (near protein probe). It was shown that the neuroprotectors Dimebon and NT-1505 affect a membrane structure. Despite the difference in membrane structures, fluidity of the lipid bilayer in time returned to control values.