High throughput sample preparation using ultrafiltration in multiwell plate format:post-sequencing cleanup of dye terminator sequencing reactions for capillary electrophoresis

摘要

A unique new method is described showing the use of microfiltration and ultrafiltration membranes in conjunction with gel permeation in 96 well plates.Using this technique it is possible to clean up DNA sequencing reactions to a level of template DNA purity that makes high DNA loading onto capillary electrophoresis (CE) slab electrophoresis.This technique repalces alcohol precipitation for sequencing reaction clean up with an easily automated process taking 1/6 the time.