Expression of genes for sectered guanyl-specific ribonuclease from Bacillus intermedius and Bacillus pumilis in Bacillus subtilis cells

摘要

Plasmids with whole genes for ribonucleases from B. intermedius and B. pumilis assembled woth the whole gene of barstar, a specific intracellular inhibitor, are constructed. The resultant plasmids pMZ55 and pMZ56 effectively express binase and RNase Bp gene in B. subtilis cells. A medium for maximum expression of RNase genes by recombinant strains is developed. The expression of binase and RNase Bp genes in B. subtilis cells is negatively regulated by exogenic inorganic phosphate.