A study of photodynamic effect of new photosensitizers on single nerve cell

摘要

We studied reactions of isolated crayfish mechanoreceptor neurons to photodynamic effects of various photo-sensitizers: methylene blue, chlorins e_6 and p_6, sulfated allumophthalocyanin Photosens, Janus green B, proto-porphyrin IX, and two derivatives of hematoporphyrin IX, Photoheme and Photosan-3. The neurons were irradiated by a helium-neon laser(632.8nm, 0.3Wt/cm~3) after 30-min photosensitization. They proved to be very sensitive to the photodynamic effect: When the cells stained by photosensitizers at nanomolar concentrations were irradiated, their firing activity underwent irreversible changes and they died. The dynamics of the firing activity of the neurons depended on the photosensitizer type and concentration. Photosens, Photoheme and chlorin p_6 proved to be the most efficient.