A Simple Process in Single Biomolecule Analysis by Total Internal Reflection Fluorescence Microscopy

摘要

Total internal reflection fluorescence microscopy (TIRFM) can produce an image and spectrum at a nanometer resolution and with a high signaloise ratio. This technique is applied to single biomolecule analysis in a growing trend. This text will introduce the basic principle of TIRFM and the techniques combined to TIRFM. In addition, the interaction of protein-nucleic acid will demonstrate the application of TIRFM to single-molecule assay in biological research.