Plasma GLP-1 Concentration and Dipeptidyl Peptidase-4 Activity in Rats Fed Partially Hydrolyzed Guar Gum are Affected, Not Plasma PYY Concentration, by Cecectomy

摘要

Objectives It studied wether cecal fermentation products affect the secretion of glucagon like peptide-1(GLP-1),peptide tyrosine tyrosine (PYY) and the activity of dipeptidyl peptidase-4 (DPP-4). Methods Sham-operated rats and cecectomized rats were fed an AIN-93 G diet with or without 5 % partially hydrolyzed guar gum (PHGG). Male Wistar rats (10 wk old) were fed an experimental diet for19 days, and olood samples were collected from the portal vein and abdominal artery.Results In rats fed the diet containing PHGCj, GLP-1 concentrations in the portal vein blood and arterial blood and DPP-4 activities in the arterial blood were lower in cecectomized rats than in sham-operated rats. PYY concentrations in the portal vein blood and arterial blood were little affected by PHGG ingestion and cecectomy. Glucose and insulin concentrations in the arterial blood were not affected by PHGG ingestion and cecectomy. In sham-operated rats fed the diet containing PHGG, the cecum tissue and cecum content weights increased compared with sham-operated rats fed a fiber-free diet. Short-chain fatty acids (SCFA) were shown that clearly increased by PHGG ingestion in sham-operated rats. It was clearly cecal fermentation products affected the secretion of GLP-1 and the activity of DPP-4 in rats fed a diet containing PHGG. Conclusions The present results show that the effect of SCFA on the metabolism of GLP-1 and DPP-4 was greater than that on PYY secretion.