Development of a quantitative PCR technique and its application to the evaluation of gene expression

摘要

The data on the development of a quantitative PCR technique are presented, including the synthesis of modified primers, the choice of conditions for the removal relation-mixture components from the amplified DNA, and the colorimetric detection of the PCR product. The application of this technique to the assessment of the level of gene expression in nonculturable bacterial forms characterized by a drastically reduced metabolism is described.