THERMOCHEMICAL CHARACTERISTICS OF 2,4-DICHLOROPHENOL DEGRADING STRAIN Pseudomonas GT241-1 OF GROWTH METABOLISM BY MICROCALORIMETRY

摘要

By using LKB-2277 Bioactivity Monitor, ampoule method, the heat output of the growth metabolism of a 2.4-dichlorophenol degrading bacteria strain, Pseudomonas strain GT241-1, has been determined at 30°C. From the thermogenic curves, it can be established that thermokinetic equation of their growth metabolism is P_t = P_t = 0 exp(k_mt), dP/dt = k_mP~1, with the order of growth metabolism n = 1. The experimental results indicate that the relationship between the matabolic power (P) and the cell concentration (C), and relationship between the metabolic power of each cell (P0) and the cell concentration can be characterized by the following thermal equation: C = a + kP, InC = a'+ k'P_0 or dC/dP_0 = KC~1. The order of the P_0-C equation n is also 1. These results are very significant in environmental sciences, biology and thermochemistry.