Chemiluminescent assay for biological substances--chemiluminescent assay for alkaline phosphatase using lucigenin and its application to enzyme immunoassay

摘要

The chemiluminescent reaction of lucigenin with various biological substances has been studied. Chemiluminescence of lucigenin is produced by the addition of organic reducing compounds to lucigenin in alkaline solution. On the basis of this reaction, we have developed highly sensitive chemiluminescent methods for the detection of enzyme immunoassay, especially using alkaline phosphatase (ALP) as a label enzyme. The detection limits of enzyme was 10(-19)-10(-20) mol per assay.