Thermal modulation of the toxicokinetics of benzo[a]pyrene in isolated hepatocytes of sablefish (Anoplopoma fimbria), black rockfish (Sebastes melanops), and chub mackerel (Scomber japonicus)

摘要

Hepatocytes from sablefish (Anoplopoma fimbria), black rockfish (Sebastes melanops) and chub mackerel (Scomber japonicus) were isolated from 11 deg C acclimated animals. The uptake, metabolism, and excretion of benzo[a]pyrene (B[a]P) in hepatocytes was measured at 6, 11 and 19 deg C. Chub mackerel hepatocyte uptake rates were significantly lower (0.012 +- 0.003 #mu#g/s per g cell ) at 11 deg C than black rockfish (0.028 +- 0.009 #mu#g/s per g cell) or sablefish (0.032 +- 0.012 #mu#g/s per g cell) hepatocytes at all temperatures. Hepatocytes metabolized B[a]p to phase I (1-8%) and phase II (92-99%) metabolites. Accumulation of phase II metabolites was lower in chub mackerel hepatocytes (0.016 +- 0.004 #mu#g/h per g cell), than black rockfish (0.052 +- 0.012 #mu#g/h per g cell), or sablefish hepatocytes (0.060 +- 0.015 #mu#g/h per g cell). Phase II metabolite accumulation increased greatest with temperature in chub mackerel hepatocytes (Q_(10) = 1.94 +- 0.30) followed by sablefish (Q_(10) = 1.65 +- 0.30), and rockfish (Q_(10) = 1.38 +- 0.30). Sablefish hepatocytes had higher excretion rates of phase II metabolites (0.010 +- 0.0023 #mu#g/h per g cell), than mackerel (0.0046 +- 0.0009 #mu#g/h per g cell) or rockfish hepatocytes (0.0029 +- 0.0008 #mu#g/h per g cell). Phase II metabolite excretion rates increased with temperature only in sablefish hepatocytes (Q_(10) = 1.67 +- 0.76). These differences in toxicokinetics may indicate distinct consequences for various species exposed to xenobiotics.