A NEW LINKER FOR SOLID-PHASE SYNTHESIS OF OLIGONUCLEOTIDES WITH TERMINAL PHOSPHATE GROUP

摘要

Synthesis of a novel cyanoethyl-type linker suitable for the solid-phase synthesis of oligo-deoxynucleotides possessing terminal 3'-phosphate group is described. Since the linker is a 2-substituted 2-cyanoethanol, the release of the synthesized oligonucleotide from the solid support is accomplished by β-elimination in the ammonia deprotection step.