Analysis of antibodies recognizing the B-epitopes formed on protein antigens after treatment with glutaraldehyde and effective method of their neutralizftion

摘要

The protein were treated with glutaraldehide and the free aldehyde groups were blocked with glycine; thus formed antigenic determinants were immunochemically characterized. The structure of the epitopes with analyzed with BSA and ovalbumin treated with glutaraldehide following the blocking of active groops with glycine and other amino acids. The products of glutaratdehyde reaction with glycine, lysyne, proline, and histidine were used as hapten-like antigens mimicking the glutaraldehyde antigenic determinants, indirect and competitive ELISA and immunoaffinity chromatography demonstrated two regions of epitope density in the structure of the glutaraldehyde determinant. The product of glutaraldehyde reaction with glycine efficiently blocked the antibodies against glutaraldehyde-treated proteins. A method of assay of the peptide-specific antibodies against of the immune complexes with the peptide-protein conjugate in presence of antibodies against a crosslinking agent is suggested usin this product.