In vitro conservation of strawberry virus indicator plants under the low temperature culture conditions.

摘要

Stolon tips of strawberry virus indicator plants were cultured on B5 medium without plant growth regulators in vials at 25鳦. After one or two months culture, these vials were transferred to low temperature condition (5鳦) and conserved for 3 monthsto 1 year. After conservation, these plantlets grew normally at 25鳦 for 3 months. These plants were then transplanted to the soil and were inoculated with strawberry vein banding caulimovirus. After inoculation, conserved plants were successfully infected with the virus, and showed virus symptoms as well as the plants normally grown in greenhouses.