Digoxigenin labeled RAPD (DIG-RAPD) analysis on genetic relations of sweet pea (Lathyrus odoratus L.) cultivars [Chinese]

摘要

Fifteen cultivars of sweet pea (Lathyrus odoratus L.) were classified using a highly sensitive RAPD method which labels amplified DNA fragments by digoxgenin (DIG-RAPD). A conventional RAPD procedure, utilizing agarose gels, detected 960 PCR fragments by using 96 primers, whereas the AE-RAPD method performed by acrylamide get electrophooresis, detected 199 fragments by 16 primers. The DIG-RAPD detected 335 PCR fragments using 16 primers indicating that it is about 2.1 times and 1.7 times more sensitive than the ordinary RAPD and AE-RAPD, respectively. Frequency of polymorphic DNA fragments generated by AE-RAPD and DIG-RAPD methods were 18.6 and 19.7%, respectively. Moreover, each UPGMA clustering analysis from ordinary RAPD, AE-RAPD and DIG-RAPD showed similar patterns. These results indicated that DIG-RAPD is an efficient RAPD method to detect polymorphic PCR fragments using fewer primers. It could also efficiently and quickly generate RAPD markers from closely related varieties, such as the sweet peas.