Surface Modification of Biomimetic PLGA-(ASP-PEG) Matrix with RGD-Containing Peptide: a New Non-Viral Vector for Gene Transfer and Tissue Engineering

摘要

RGB- containing pelatin (K16- GRGDSPC), characterized as non-viral gene vectors, was fabricated to modify the surface of PLGA-[ASP-PEG] matrix, which offered the foundation for gene transfer with porous matrix of gene activated later. Peptide was synthesized and matrix was executed into chips A, B and chip C. Chip C was regarded as control. Chips A arid B were reacted with cross- linker. Then chip A was reacted with peptide. MS and HPLC were used to detect the MW and purity of peptide. Sulphur, existing on the surface of bi-omaterials, was detected by XPS. The purity of un- reacted peptide in residual solution was detected by a spectro-photometer. HPLC shows that the peptide purity was 94 percent - 95 percent, and MS shows that the MW was 2 741.3307, XPS reveals that the binding energy of sulphur was 164 eV and the ratio of carbon to sulphur (C/S) was 99.746 : 0.1014 in reacted chip A. The binding energy of sulphur in reacted chip B was 164 eV and 162 eV, CIS was 99.574:0.4255, and there was no sulphur in chip C. Peptide was manufactured and linked to the surface of bio-mimetic and 3-D matrix, which offered, the possibilities for gene transfer and tissue engineering with this new kind of nan- viral gene vector.