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首页> 外文期刊>Journal of Zoo and Wildlife Medicine >Sero-epizootiologic survey of Trypanosoma brucei in Kenyan nonhuman primates
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Sero-epizootiologic survey of Trypanosoma brucei in Kenyan nonhuman primates

机译:肯尼亚非人类灵长类动物布鲁氏锥虫的血清流行病学调查

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摘要

Blood samples were collected from 121 individuals of three species of wild-caught nonhuman primates from Kenya, including African green monkeys (Cercopithecus aethiops), Syke's monkeys (C. mitis), and olive baboons (Papio cynocephalus anubis), and were examined for circulating Trypanosoma brucei and for T. brucei antigen and anti-trypanosome antibody. Indirect antibody enzyme-linked immunosorbent assay detected titers of anti-T. brucei antibodies in 13 of the primates sampled, and field-oriented latex agglutination test detected invariant T. brucei antigens in 10 (8.3%) of the primates. However, no trypanosomes were visible in blood smears, on wet blood films, or by buffy coat technique, nor were they demonstrable in a subset of C. aethiops individuals that were studied using mouse subinoculation.
机译:从肯尼亚的三种野生非人类灵长类动物的121个人中采集了血液样本,包括非洲绿猴(Cercopithecus aethiops),赛科猴(C. mitis)和橄榄狒狒(Papio cynocephalus anubis),并检查了其循环情况布氏锥虫和用于布氏锥虫抗原和抗锥虫抗体。间接抗体酶联免疫吸附测定法检测抗T的效价。在13个灵长类动物中采集了brucei抗体,而场定向乳胶凝集测试则在10个灵长类动物中(8.3%)检测到了布鲁氏杆菌不变抗原。但是,在血涂片,湿血膜或通过血沉棕黄层技术上都看不到锥虫体,在用小鼠亚接种研究的拟南芥嗜酸子囊菌个体中也没有表现出锥虫体。

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