Microcalorimetric study of the effect of artesunate on the growth metabolism of mitochondria isolated from rat liver

摘要

In this study, themetabolic thermogenic curves of mitochondria isolated fromrat liver tissuewere drawn, and the effects of artesunate on metabolic activity of mitochondria were studied using TAM Air Isothermal Microcalorimeter. After isolation from the rat liver, mitochondria still have metabolic activity and can live for a week with appropriate nutrients. With the addition of artesunate (7.5 lM/mL), the metabolic activity ofmitochondria was completely lost at this concentration. From the thermogenic curves, we obtained the thermokinetic equations under different conditions: T_(max) = 2512.24 + 1761.53c, R = 0.9375; and Tlag (min) = 707.26 + 285.17c, R = 0.9846. The results showed that artesunate was a mitochondria-targeted drug having obvious effects on mitochondrial metabolism.