Detection of banana bract mosaic virus (BBrMV) in Musa spp. by serological and molecular techniques

摘要

Banana bract mosaic virus (BBrMV) isolates from different parts of the country were transmitted to different Musa spp. by sap and aphid inoculation. PCR products (DINA) of file different isolates exhibiting varied symptom types on different varieties were tested by Southern blot to determine differences in isolates. Detection using serology and molecular techniques were also used on the different isolates to test the efficiency of the two techniques. The different isolates of BBrMV from sap and aphid transmission using two aphid species did not show any differences as supported by Southern blot test. On symptomatic plants, routine detection can be done by enzyme linked immunosorbent assay (ELISA). Immunocapture reverse transcriptase polymerase chain reaction (IC-RT-PCR) would be more sensitive in detecting low concentration of the virions.