ISOLATION AND TRANSMISSION OF TOMATO LEAF CURL VIRUS IN THE PHILIPPINES

摘要

A virus causing the leaf curl disease in tomato was isolated and maintained in tomato. The putative tomato leaf curl virus (TLCV) was transmitted by whiteflies in a circulative manner. The virus was transmitted after 2 hr acquisition access period and1 hour inoculation access period. It has a latent period of 24 hr in the vector and inoculated plants developed the symptoms 2-3 weeks after inoculation. TLCV can be transmitted by the tissue implantation method but not mechanically transmissible. The host range of the virus include Datura ftrnnioniuni, Nicotiatia glittinosa, N. tabacum, and N. syhvstris. Different tomato entries reacted with varying degrees of leaf curls, crinkling, leaf size reduction and stunting. The TLCV isolates positively reacted to monoclonal antibodies and to nonradooactive DNA probes specific for tomato yellow leaf curl virus from other I countries. The results confirm the occurrence of the B. tabaci transmitted gemmivirus TLCV in the Philippines.