Therapeutic efficacy of isometamidium hydrochloride against experimentally induced Trypanosoma evansi infection in rats

摘要

The generic drug, Isometamidum-HCl was evaluated against Trypanosoma evansi infection in rats. Fift> Sprague-Dowley rats (b.wt 150-200 gms) of either sex were divided into five equal groups. Rats of (Jr. II to V were inoculated intraperitoneally with0.25 ml (104 numbers of parasites) of T. evansi infected rat blood diluted in equal volume of Alsever's solution and the Gr. I served as healthy control. On appearance of teeming level of parasitaemia rats of Gr.-III, IV and V were treated (IIP) with Isometamidium-HCl @ 0.25 mg, 0.5 mg and 1.0 mg/Kg b.wt, respectively. The drug was re-administered on 15 DPT at higher doses (2.0 mg/ Kg b.wt) when parasitaemia reappeared in the treated group of rats. The infection resulted in 100 per cent mortality by 7 DPT in the infected untreated rats (Gr. II) and Isometamidium-HCl was 40, 70 and 90 per cent effective @ 0.25 mg, 0.5 mg and 1.0 mg/Kg b.wt, respectively. Re-treatment of the resurgent infection with 2.0 mg/Kg b.wt exerted 100 per cent trypanocidal efficacy. The drug @ 0.5 mg and 1.0 mg/Kg b.wt resulted (P<0.1) in remission of body temperature on 1 DPT. Isometamidium-HCl treatment caused a significant (P<0.01) negative impact on the body weight of treated rats.