Evaluation of the analytical performance of an enzyme immunometric assay (EIA) designed to measure endogenous thyroid-stimulating hormone (TSH) in canine serum samples.

摘要

A commercial enzyme immunometric assay (EIA) for endogenous thyroid-stimulating hormone (cTSH) concentration was validated using serum samples form 6 dogs with suspected hypothyroidism, 10 apparently healthy dogs and a control group of 8 laboratoryBeagles. The assay showed intra- and inter-assay coefficients of variation (CV) of 5 and 11%, respectively. The assay maintained linearity under dilution and recovered added cTSH in a linear and proportional manner. The limit of detection (0.03μg/litre)was well below the values measured in serum from dogs with primary hypothyroidism (median value 1.52μg/litre). The assay clearly distinguished dogs with primary hypothyroidism from clinically healthy dogs (P = 0.0004). It is concluded that the assay was acceptable.