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Quasi-physical phase compensation in digital holographic microscopy

机译:数字全息显微镜中的准物理相位补偿

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摘要

In digital holographic microscopy, if an optical setup is well aligned, the phase curvature introduced by the microscope objective (MO) together with the illuminating wave to the object wave is a spherical phase curvature. It can be physically compensated by introducing the same spherical phase curvature in the reference beam. Digital holographic microscopy setups based on the Michelson interferometric configuration with MO and an adjustable lens are presented, which can well perform the quasi-physical phase compensation during the hologram recording. In the reflection mode, the adjustable lens serves as both the condensing lens and the compensation lens. When the spatial frequency spectra of the hologram become a point spectrum, one can see that the phase curvature introduced by imaging is quasi-physically compensated. A simple plane numerical reference wavefront used for the reconstruction can give the correct quantitative phase map of the test object. A theoretical analysis and experimental demonstration are given. The simplicity of the presented setup makes it easy to align it well at lower cost.
机译:在数字全息显微镜中,如果光学装置对准良好,则由显微镜物镜(MO)引入的相曲率与照明波一起入射到物波就是球面相曲率。通过在参考光束中引入相同的球形相位曲率,可以对其进行物理补偿。提出了基于具有MO和可调节透镜的迈克尔逊干涉测量配置的数字全息显微镜设置,它们可以在全息图记录期间很好地执行准物理相位补偿。在反射模式下,可调透镜既用作聚光透镜又用作补偿透镜。当全息图的空间频谱变成点频谱时,可以看到成像引入的相曲率是准物理补偿的。用于重建的简单平面数字参考波前可以给出测试对象的正确定量相位图。进行了理论分析和实验证明。所提供设置的简单性使其易于以较低的成本很好地对准。

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