Silicon-29 NMR evidence of a transient hexavalent silicon complex in the diatom Navicula pelliculosa

摘要

Cultures of the freshwater diatom Navicula pelliculosa were synchronized by silicon starvation, fed a ~(29)Si-enriched silicate solution, and then studied yb ~(29)Si NMR spectroscopy. Two NMR resonances could be detected reproducibly, the ever-present orthosilicic acid peak at -71 ppm and a weak signal at -131.5 ppm that was only seen after the diatoms had been allowed six hours to accumulate the ~(29)Si. When the initial culture medium was enriched in ~(15)N, the -131.5 ppm resonance narrowed significantly which, taken with the peak's unique chemical shift, implies the existence of an organosilicon complex containing hexavalent silicon coordinated of at least one nitrogen. The signal is the first direct evidence of an organosilicon complex formed during the life cycle of an organism.