Design of poly-L-methionine–gold nanocomposit/multi-walled carbon nanotube modified glassy carbon electrode for determination of amlodipine in human biological fluids

摘要

The present paper describes a sensitive electrochemical detection of amlodipine (AMLO) at the poly-Lmethionine–gold nanoparticles/multi-walled carbon nanotube modified glassy carbon electrode (PLM–GNPs/MWCNTs/GCE) by differential pulse voltammetry (DPV) technique at physiological pH 7.12. Cyclic voltammetry results demonstrate that the proposed electrode shows excellent electrocatalytic activity toward oxidation of AMLO. Kinetic parameters of the electrochemical reaction are calculated, and analytical variables such asMWCNT volumes, drug accumulation time, electropolymerization cycles and pH values are also optimized. Under optimal conditions, the linear range covering from 5 nM to 2.5 μM along with detection limit of 1 nM is obtained.Moreover, this method is successfully used to detect AMLO in pharmaceutical samples and biological fluids of a dosage received by the volunteer.