Substrate Specificity and Stability of Layered Bio-lnorganic Nanocomposites

摘要

Substrate specificity of layered nanocomposites of global myoglobin(Mb)-magadiite were inves-tigated for different substrates, including aniline, o-phenylenediamine, phenol, p-aminophenol, o-aminophenol, p-methoxyphenol and o-methoxyphenol. The results showed that the catalyzing effect of immobilized Mb was the highest for o-phenylenediamine (352.1%), phenol (138.7%), o-methoxyphenol (96.7%), o-aminophenol (94.6%), p-aminophenol (72.6%), aniline (60.4%), p-methoxyphenol (35.1%), respectively, when the effect of catalyzing for free Mb was deter-mined as 100%. Based on the kinetic analysis, the enhancement of catalytic activity under o-phenylenediamine was about 5 and 8 times increases in Vmax and kat values for the immobilized Mb, respectively, compared to that of free Mb. When Mb–magadiite nanocomposites powders were kept for two months at room temperature, the enzyme activity of the immobilized Mb decreased only about 8.6% when o-phenylenediamine was used.