Existence of co-sexuality in palmyrah palm and study of relationship between monoecious and dioecious palms using molecular markers.

摘要

A study was conducted to examine the relationship between monoecious and dioecious palmyrah palm (Borassus flabellifer) at DNA level and sex determination of 7 seedlings generated from monoecious palm using the male sex-linked random amplified polymorphic DNA marker. Leaf samples were collected for DNA extraction from the monoecious palm located at Undeswarapuram (Andhra Pradesh, India) and its 7 progenies planted in 2003 and maintained at Andhra Agricultural University, Pandirimamidi. Immature leaf samples from pistillate and staminate palms were collected from Kasaragod (Kerala, India) to serve as the control. The male-specific band of size 600 bp was present in monoecious palm as well as in 6 out of the 7 progenies. One progeny was possibly a female (palm No. 7 at Pandirimamidi Station) and the rest of the progenies (palm No. 4, 5, 8, 9, 11 and 12) may be either monoecious or males. No marker has been identified as yet to differentiate monoecious and males.