New primers for amplification of the planctomycetes 16S rRNA gene from environmental samples.

摘要

Although the 16S rDNA primer set Pla-46-F/1392-R is often used to explore the planctomycetes community, the universal reverse primer 1392-R limits the capability of the forward primer Pla-46-F. Here we report two new reverse primers (Pla-1097-R and Pla-1368-R), which greatly promote the potential ability of the forward primer Pla-46-F, both theoretically and experimentally. The tests with field samples showed that PCR induced by the new primer sets both reduced mismatches to non-planctomycetes and supplemented the diversity of planctomycetes, particularly increasing the numbers of planctomycetes genera in both seawater and sediment samples. Our results strongly suggest that the diversity of planctomycetes revealed by the previously used primer set Pla-46F/1392-R has been underestimated.