Liu, L., Zhang, L., Wang, Y., Tian, L., Gong, X., Li, Q.A strategy for quality control of the fresh rejuvenated fruits of Juglans mandshurica based on cytotoxicity screening and efficacy-fingerprint analysis

摘要

The fresh rejuvenated fruits of Juglans mandshurica (QLY), known as a traditional Chinese medicine, has long been used in clinical practice for the treatment of cancer. Nevertheless, the existing quality control method seems insufficient to reflect the effective constituents of QLY and guarantee its quality, safety, and clinical efficacy. In this research, the cytotoxicities of different solvent fractions of QLY against human colon carcinoma cell line LS-174 T were estimated by MTT assay. Then, the efficacy-fingerprints of QLY with predominate cytotoxic activity were established by high performance liquid chromatography. In the fingerprints, fifteen peaks were selected as the common peaks to evaluate the similarities of 20 batches of samples from different sources. Relationships between cytotoxicity and chromatographic profiles were also investigated. The results indicated that the levels of peaks 4, 5, and 6 correlated closely with cytotoxicity. It is proposed that the efficacy-fingerprint with key biomarkers (peaks 4 and 5) should be adopted for improving the quality control of QLY. The developed method by having a combination of cytotoxicity screening and efficacy-fingerprint analysis could be an easy and fast analytical approach to find out effective components for quality control of QLY and its related traditional Chinese medicinal preparations.