Simultaneous determination of di(2-ethylhexyl)phthalate, mono(2-ethylhexyl)phthalate, and phthalic acid migrating from gamma-ray irradiated polyvinyl chloride sheet by liquid chromatography-tandem mass spectrometry

摘要

The aim of the present study was to establish a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of di(2-ethylhexyl) phthalate (DEHP), mono(2-ethylhexyl)phthalate (MEHP), and phthalic acid (PA). In the proposed method, the limits of detection of DEHP, MEHP, and PA were 5, 0.5, and 1 ng/mL, respectively, and the limits of quantification with standard solutions were 20, 2, and 5 ng/mL, respectively. Intra- and interday assays showed good accuracy and repeatability. The recoveries of DEHP, MEHP, and PA from respective extraction solvents ranged from 98.9 to 104.2% (relative standard deviation was below 10.3%). DEHP and its breakdown products migrating from gamma-ray irradiated polyvinyl chloride (PVC) sheets were determined simultaneously since DEHP is easily eluted from PVC medical devices. DEHP migration was noted from both gamma-ray irradiated and control PVC sheets. Compared with the migration from the control PVC sheet, MEHP showed significant migration from the gamma-ray irradiated PVC sheet. In contrast, PA migration was noted only from the gamma-ray irradiated PVC sheet.