Determination of triflusal in human plasma by high performance liquid chromatography with automated column switching system

摘要

An analytical method of triflusal in human plasma was devel- oped using semi-microbore HPLC equipped with automated col- umn switching system. p- Toluic acid which is the structural ana- logue of triflusal, was used as an internal standard and 2 M HCI was employed as a stabilizer. The load phase and mobile phase were prepared using acetonitrile and 20 mM KH2PO4 with the vol- ume ratios of 10:90 (pH 2.5) and 43:57 (pH 2.3), respectively. The signals were monitored by a UV detector at 275 nm with flow-rate of load phase, 500 lJ.L/min, and mobile phase, 100 lJ.L/min, respectively. The retention time of triflusal and p-toluic acid was about 20.2 min and 16.4 min, respectively. The detec- tion limit. of triflusal in human plasma was 0.01 lJ.g/mL and the 1imit of quantitative analysis was 0.05 lJ.g/mL. The accuracy of the assay was from 97.76% to 116.51% while the intra-day and inter-day coeft'icient of variation of the same concentration range was less than 15%. This analytical method demonstrated excel- lent sensitivity, reproducibility, specificity, and speed using the plasma sample. This method could be successfully applied to evaluate the bioavailability of triflusal in human subjects without time-consuming sample clean-up after oral administration of low dose.