APPLICATION OF THE IODINE-AZIDE PROCEDURE FOR THE DETECTION OF GLYCINE, ALANINE, AND ASPARTIC ACID IN PLANAR CHROMATOGRAPHY

摘要

The application of iodine-azide reaction for the determination of non-sulphur amino acids (glycine, alanine, and aspartic acid) transformed into PTC-derivatives with phenyl isothiocyanate in TLC and HPTLC, is described. The developed plates were sprayed with a freshly prepared mixture of sodium azide, adjusted to a proper pH, and starch solution, and exposed to iodine vapour for 5 seconds. The detection limits were established at the pmole level. The factors depending on the detection limits were described. A comparison of iodine-azide test reactions with other procedures is presented. The developed method was applied to detection of aspartic acid in a drug. The possibility of detection of amino acid mixtures was demonstrated.