LANTHANIDE LUMINESCENCE DETECTION OF BLEOMYCINS AND NALIDIXIC ACID

摘要

Bleomycins and nalidixic acid transfer energy to Tb(III) Subsequent emission from Tb(III) provides for a selective and sensitive detection method. Temperature studies indicate that energy transfer for both compounds involves an intramolecular process. The efficiency of reversed-phase separations of bleomycins is improved by adding Tb(III) to the mobile phase. Tb(III) luminescence detection is compatible with a wide range of mobile phases suitable for the separation of nalidixic acid. Detection limits for bleomycin A(2) and nalidixic acid are 3 x 10(-6) M and 4 x 10(-7) M respectively.