首页> 外文期刊>Journal of Pure & Applied Microbiology >Effect of Fermentation Temperature on Mannatide Production by alpha-hemolytic StreptococcusAU Xu, Cui Zhang, Jialan Xiong, Qiu Chen, Yali Gao, Mengxiang (mxgao0398@163.com)
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Effect of Fermentation Temperature on Mannatide Production by alpha-hemolytic StreptococcusAU Xu, Cui Zhang, Jialan Xiong, Qiu Chen, Yali Gao, Mengxiang (mxgao0398@163.com)

机译:Effect of Fermentation Temperature on Mannatide Production by alpha-hemolytic StreptococcusAU Xu, Cui Zhang, Jialan Xiong, Qiu Chen, Yali Gao, Mengxiang (mxgao0398@163.com)

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摘要

Temperature is one of the most important environmental factors for cell growth and product formation. Batch microbial mannatide fermentations by alpha-hemolytic Streptococcus H1S-33 at various temperatures, ranging from 28 to 39 degrees C, were studied. The lag time was shorter and the specific cell growth rate and specific mannatide production rate in the early stages of growth were higher at 30 degrees C or higher temperature. The average cell growth rate was higher and the biomass reached the maximum earlier than other temperature at 37 degrees C during fermentation 0-11 h and declined obviously after 11 h, but they remained at a higher level at 33 degrees C after 11 h. Therefore the two-phase temperature control strategy was presented. The temperature control strategy was that the temperature was 37 degrees C during 0-11 h, it Was adjusted to 33 degrees C with the descending 1 degrees C an hour after 11 h. The biomass (dry cell weight) and mannatide production yield reached 9.24 g.L-1 and 1.16 g.L-1 at the variable temperature, respectively, which were higher than those at other temperatures.C1 Gao, Mengxiang; 266 Jingmi Rd, Jinzhou 434025, Hubei, Peoples R ChinaSC Biotechnology & Applied Microbiology
机译:温度是细胞生长和产物形成的最重要的环境因素之一。研究了α-溶血链球菌H1S-33在28至39摄氏度的不同温度下分批发酵微生物甘露聚糖的情况。在30℃或更高的温度下,滞后时间较短,并且在生长早期的比细胞生长速率和比甘露糖苷产生速率更高。在发酵0-11小时内,平均细胞生长速率较高,且生物量在37摄氏度时比其他温度更早达到最大值,并在11小时后明显下降,但在11小时后它们在33摄氏度下保持较高水平。因此提出了两相温度控制策略。温度控制策略是在0-11小时内温度为37摄氏度,在11小时后每小时将温度调节为33摄氏度,然后每小时下降1摄氏度。高温下生物量(干细胞重)和甘露聚糖产量分别达到9.24 g.L-1和1.16 g.L-1,高于其他温度。中华人民共和国湖北省锦州市经密路266号434025

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