The Chemical Modification of Cellulase from Locale Bacteria Isolate Bacillus subtilis ITBCCB148 with Glyoxylic Acid

摘要

This research aims to increase the stability of cellulase obtained from Bacillus subtilis ITBCCB148 using glyoxylic acid as modifier. To achieve this aim, the research phases performed were production, isolation, purification and chemical modificationon cellulase and were followed by characterization of the purified (native) and modified enzyme to know the stability increase of the enzyme. The result showed that the purified enzyme has optimum pH 6, temperature optimum 60°C; k. = 0,066 min.~(-1); t_(1/2) = 10.50 min.; and AG. = 100.7330 kj/mol, while the modified enzymes with glyoxylic acid with modification degree of 70.54; 78.68; 86.43% have similar optimum pH and temperature with the native enzyme, but the thermal stability of the three modified enzymes were k. = 0.031; 0.033; and 0.037 min.t_(1/2) = 22.35; 21.00; and 18.72 minutes, and AG. = 102.8253; 102.6522; and 102.3354 kj mol '. respectively. The chemical modification has been able to increase the thermal stability of the modified enzymes 1.8 - 2.1 times more than the native one.