Indigenous Porcine Gastro-intestinal Tract as Veritable Source of Probiotic IsolatesAU Langa, Rashwahla Lesiba Sydwell Makete, Goitsemang Bruwer, Marietjie Mofokeng, Dikonketso Shirley-May Goitsema, Christa Aiyegoro, Olayinka Ayobami (AiyegoroO@arc.agric.za)

摘要

Piglets weaning age is usually fast tracked to between 3-4 weeks for economic reasons. This burdens the immature gastro-intestinal tract of the weaned piglets with associated post weaning diarrhoeal syndromes (PWDs). In the European Union, the use of antibiotics as growth promoter in animal feed has been banned since 2006, so, in an attempt to finding alternative to the management of PWDs, this research was designed to isolate and characterize probiotic from indigenous South African Windsnyer pig breed. Four isolates namely: Lactobacillus reuteri ZJ625, Lactobacillus reuteri VB4, Lactobacillus salivarius ZJ614 and Streptococcus salivarius NBRC 13956 were characterized based on their probiotic attributes. The isolates resisted bile salt up to 1.2%v/v and survive at low pH of 3 with OD590 values ranging from 0.45 to 1.26 after 12 h incubation. From the adherence and survival assay, the average viable cell count after 6h incubation is 5.15 x 10(8) cfu/mL, with the highest viable count of 9.4 x 10(8) cfu/mL observed in Lactobacillus salivarius ZJ614 after 6 h of incubation. These results revealed probiotics that could be used in pig husbandry, efforts are on-going to further characterise these probiotics, develop a porcine gastro-intestinal model and ultimately establish an in vivo protocol.C1 Aiyegoro, Olayinka Ayobami; Anim Prod Inst, Agr Res Council, Gastrointestinal Microbiol and Biotechnol Unit, Private Bag X02, ZA-0062 Pretoria, South AfricaSC Gastroenterology & Hepatology; Pharmacology & Pharmacy; Agriculture