Classical Densitometry and Videoscanning in a New Validated Method for Analysis of Candesartan and Losartan in Pharmaceuticals

摘要

Classical densitometry and videoscanning were compared for a new TLC method of quantitative analysis of two AT, receptor antagonists, candesartan and losartan, in Pharmaceuticals. Chromatography was performed on silica gel with l,4-dioxane-hexane-99% formic acid 5:5:0.1 (v/v) as mobile phase. Classical densitometry was performed at the wavelengths of maximum absorption of candesartan (258 nm) and losartan (243 nm) whereas videoscanning was performed at 254 nm for both drugs. Compact spots were obtained for candesartan (R_F 0.47 +- 0.01, mean +- SD) and losartan (R_F 0.35 +- 0.01). Calibration plots were constructed in the range 0.2-1.4 ug per band for the both drugs and were linear with good correlation coefficients - 0.9997 and 0.9981 for candesartan, and 0.9986 and 0.9982 for losartan, for densitometry and videoscanning, respectively. The methods were validated for robustness, precision, accuracy, and specificity in relation to excipients present in the respective formulations. Finally, the methods were compared statistically in respect of robustness, precision and accuracy.