SUSCEPTIBILITY LEVEL OF THE COLORADO POTATO BEETLE (LEPTINOTARSA DECEMLINEATA SAY) TO CHLORPYRIFOS AND ACETAMIPRID IN POLAND AND RESISTANCE MECHANISMS OF THE PEST TO CHLORPYRIFOS

摘要

Nowadays, neonicotinoids play an essential role in the control of the Colorado potato beetle (CPB) in Poland. Taking into consideration that CPB shows some resistance to pyrethroids and the main role of oxidative metabolism in this resistance, research was conducted to estimate CPB susceptibility level to chlorpyrifos and acetamiprid. The results pointed to a lack of CPB resistance to acetamiprid and a weak susceptibility level to chlorpyrifos by the CPB. For this reason, the second part of the experiment was aimed at detecting the resistance mechanisms of the CPB to chlorpyrifos. Results showed that none of the tested enzyme groups (oxidases, esterases and glutathione transferases) are the reason for CPB resistance to chlorpyrifos. The experiments revealed an increase in the beetles survival after adding oxidative enzyme blocker to chlorpyrifos.