PHYTOCHROME FROM PROTONEMAL TISSUE OF THE MOSS CERATODON PURPUREUS

摘要

Extracts of protonemal filaments of the moss Ceratodon purpureus were analysed for phytochrome by photoreversibility measurements and immunoblotting. Phytochrome content in vivo is light dependent; following transfer from white light to darkness, phytochrome accumulated over a 7-day period, whereas upon transfer from darkness to light, phytochrome content decreased rather slowly. In vitro, part of the Ceratodon phytochrome pool showed a rapid (< 10 min) dark reversion from Pfr to Pr. A Pr minus Pfr difference spectrum of partially-purified phytochrome showed an absorbance maximum at 660 nm and a minimum at 722 nm.