Influence of phosphorus limitation on toxicity and photosynthesis of Alexandrium minutum (Dinophyceae) monitored by in-line detection of variable chlorophyll fluorescence

摘要

The effects of phosphorus (P) limitation on growth, toxicity, and variable chl fluorescence of Alexandrium minutum were examined in batch culture experiments. Cell division was greatly impaired in P-limited cultures, but P spiking of these cultures after 9 days stimulated high levels of cell division equivalent to P-replete cultures. The cellular concentration of paralytic shellfish toxins was consistent over the growth cycle of control cultures from lag phase into logarithmic growth phase, with toxins repeatedly lost to daughter cells during division. The low level of cell division in P-limited cultures resulted in a 10-fold increase of cellular toxin compared with controls, but this dropped upon P spiking due to increased rates of cell division. The history of phosphorus supply had an important effect on toxin concentration, with the P-limited and the P-spiked cultures showing values 2-fold higher than the P-replete cultures. Toxin profiles of the A. minutum strain used in these experiments were dominated by the N-1 -hydroxy toxins, gonyautoxins (GTX) GTX1 and GTX4, which were approximately 40 times more abundant than their analogues, GTX2 and GTX3, in P-limited cultures. The dominance of the N-1 -hydroxy toxins increased significantly in control cultures as they advanced through logarithmic growth. In-line measurements of the variable chl fluorescence of light-adapted cells indicated consistent photochemical efficiency under P-replete conditions. P limitation induced a drop in fluorescence-based photochemical efficiency that was reversible by P spiking. There was an inverse linear relationship between in-line fluorescence and cell toxin quota (r = -0.88). Monitoring fluorescence in-line may be valuable in managing efficient biotechnological production of toxins. [References: 38]