Electrochemical study on the behavior of Morin and its interaction with DNA.

摘要

Voltammetric behavior of Morin was studied in 0.1M HAc-NaAc+50mM KCl (pH 3.4) solution at glassy carbon electrode (GCE) using cyclic voltammetry (CV). Morin showed an irreversible anodic peak at 0.720 V in CV which was involving two electrons and two protons. Also, the interaction of Morin with double-stranded calf thymus DNA (ctDNA) was studied by CV at GCE with an irreversible electrochemical equation. As a result of reaction with ctDNA, the voltammetric peak of Morin was a position shift and the peak current decreased. The diffusion coefficients of both free and binding Morin (D(f)=1.1,086 x 10(-7)cm(2)s(-1) and D(b)=8.2,544 x 10(-9)cm(2)s(-1)), binding constant (K=1.7,765 x 10(7)cm(3)mol(-1)), and binding site size (s=0.8,510) of the Morin-DNA complex were obtained simultaneously by non-linear fit analysis. The results demonstrate that Morin can bind to ctDNA in 0.1M HAc-NaAc+50mM KCl (pH 3.4) solution and the ring B of Morin intercalates between the DNA base pairs.