The role of uncoupling protein 2 and 3 genes polymorphism and energy expenditure in obese Indonesian children

摘要

Aim: Uncoupling protein (UCP) genes, which may contribute to energy metabolism in mitochondria, may be involved in the pathogenesis of obesity. We analyzed the differences in energy expenditure between single nucleotide polymorphisms (SNPs) UCP3 - 55C/T , UCP3 Y210Y , and UCP2 A55V among Indonesian children. Methods: The study included 76 schoolchildren (36 obese and 40 healthy; mean age, 12.8 years) in Semarang, Indonesia. Body composition was measured by bioelectrical impedance analysis; resting energy expenditure (REE) by indirect calorimetry; physical activity by uniaxial accelero meter; and total energy expenditure (TEE) by the equations extrapolated from REE and physical activity. UCP3-55C/T , UCP3 Y210Y , and UCP2 A55V were examined by restriction length fragment polymorphism analysis. Results: The TEE of the subjects with the T/T genotype at UCP3-55C/T after adjusting for fat-free mass (63.2 ± 7.2 kcal/ kg/day) and T/T at UCP2 A55V (62.8 ± 5.6 kcal/kg/day) was lower than that of the subjects with the C/C and C/T genotypes (p < 0.05). The REE of the subjects with these T/T geno types tended to be lower than that of the subjects with C/C and C/T (p = 0.05). No significant differences in REE or TEE were found between the UCP3 Y210Y genotypes. Conclusions: The subjects with the T/T genotypes of UCP3-55C/T or UCP2 A55V had lower TEE than those with other genotypes.