Study on the Tissue Culture of Coleus blumei

摘要

This paper studied on the way of Coleus blumei, the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine (6-BA), alpha-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA). The optimalconditions of callus induction from explants were achieved on the medium containing 6-BA (2.0 mg-L~(-1)) and NAA (1.0 mg-L~(-1)). Shoot tips were induced on the medium containing 6-BA (4.0 mg-L~(-1)) and NAA (0.5 mg-L~(-1)). The same media conditions were found suitable for shoot multiplication, we multiplied shoots rooted best on 1/2 MS medium supplemented with IB A (0.1 mg-L~(-1)).