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首页> 外文期刊>Journal of molecular catalysis, B. Enzymatic >Expression of organophosphorous hydrolase in Escherichia coli for use as whole-cell biocatalyst
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Expression of organophosphorous hydrolase in Escherichia coli for use as whole-cell biocatalyst

机译:用作全细胞生物催化剂的有机磷水解酶在大肠杆菌中的表达

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摘要

Among the various removal strategies against neurotoxic organophosphorus (OP) compounds, a live catalyst using whole-cell microorganisms has been considered as a useful scavenger with the virtue of cost-effectiveness and elimination efficiency. To over the catalytic activity by wild-type isolates, the expression of organophosphorus hydrolase (OPH) has been attempted in Escherichia coli for the extended detoxification efficacy to OP chemicals. However, early studies had unsatisfactory results, like low enzyme production levels due to the formation of insoluble inclusion bodies from the recombinant enzyme produced within the cells, and a bottleneck caused by the cell membrane of gram-negative strain £ coli acting as a permeability barrier to substrate diffusions. To date, various approaches have been suggested as effective solutions for overcoming these limits. This review will outline current studies, and their critical findings, on the successful expression of OPH in £ coli for the effective recombinant £ coli whole-cell biocatalysts.
机译:在针对神经毒性有机磷(OP)化合物的各种去除策略中,利用全细胞微生物的活性催化剂凭借成本效益和消除效率的优势被认为是有用的清除剂。为了超过野生型分离物的催化活性,已经尝试在大肠杆菌中表达有机磷水解酶(OPH),以扩大对OP化学品的解毒功效。然而,早期研究的结果并不令人满意,例如由于细胞内产生的重组酶形成不溶性包涵体而导致酶的生产水平低下,以及革兰氏阴性菌株£大肠杆菌的细胞膜作为通透性屏障而引起的瓶颈。到衬底扩散。迄今为止,已经提出了各种方法作为克服这些限制的有效解决方案。这篇综述将概述有效的重组大肠杆菌全细胞生物催化剂在OPH在大肠杆菌中成功表达的最新研究及其关键发现。

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