Reproducible MRI measurement of adipose tissue volumes in genetic and dietary rodent obesity models.

摘要

PURPOSE: To develop ratio MRI [lipid/(lipid+water)] methods for assessing lipid depots and compare measurement variability with biological differences among lean controls (spontaneously hypertensive rats [SHRs]), dietary obese rats (SHR-DOs), and genetic/dietary obese rats (SHROBs). MATERIALS AND METHODS: Images with and without chemical shift-selective (CHESS) water suppression were processed using a semiautomatic method that accounts for relaxometry, chemical shift, receive coil sensitivity, and partial volume. RESULTS: Partial volume correction improved results by 10% to 15%. Over six operators, volume variation was reduced to 1.9 mL from 30.6 mL for single-image-analysis with intensity inhomogeneity. For three acquisitions on the same animal, volume reproducibility was <1%. SHROBs had six times more visceral and eight times more subcutaneous adipose tissue than SHRs. SHR-DOs had enlarged visceral depots (three times larger than those in SHRs). SHROBs had significantly more subcutaneous adipose tissue, indicating a strong genetic component to this fat depot. Liver ratios in SHR-DO and SHROB were higher than in SHR, indicating elevated fat content. Among SHROBs, evidence suggested a phenotype SHROB* having elevated liver ratios and visceral adipose tissue volumes. CONCLUSION: Effects of diet and genetics on obesity were significantly larger than variations due to image acquisition and analysis, indicating that these methods can be used to assess accumulation/depletion of lipid depots in animal models of obesity.